Impact of Metformin and Pioglitazone on Serum Level of Tumor Necrosis Factor-Alpha and Lipid Profiles during Implantation Window in Diabetic Rats

Objective Objectives: The present study was designed to evaluate the serum lipid profile and Tumor Necrosis Factor alpha (TNF-ɑ) level in diabetic rats at the time of implantation. Type 2 diabetes mellitus (T2DM) could affect various systems, including innate immune system and causes chronic low-grade inflammation which increases the level of TNF-ɑ. Furthermore, T2DM is often accompanied by impaired lipid profile. Metformin and pioglitazone are used as the first and second line of treatment for T2DM. MaterialsAndMethods Thirty-five adult virgin female wistar rats weighting 175-225 g were randomly categorized into five groups: (1) control (2) sham (3) Nicotinamide (NA)+Streptozotocin (STZ) induced T2DM, (4) Diabetic + pioglitazone (20 mg/kg/day for 28 days per oral) and (5) Diabetic + metformin (100 mg/kg/day for 28 days per oral). At the time of implantation, TNF-ɑ level in serum of rats was measured by ELISA kit. Glucose was measured using photometric method and lipid profiles were calculated by enzymatically methods. Results The level of TNF-ɑ in the diabetic group was significantly higher than other groups (P